Virtual Education Project Pakistan (VEPP) is going to organize a free online course entitled “NMR Spectroscopy of Biological Molecules” by Prof. Dr. Ioannis P. Gerothanassis commencing from April 19, 2016. This course fulfills one credit-hour course requirements. On-site students at the ICCBS will have 14 hours of formal class.
Introduction of the Speaker
Prof. Dr. Ioannis P. Gerothanassis is Professor at the Department of Chemistry, University of Ioannina (Uol). He is Former Rector of the University of Ioannina (Uol), also the Vice Chairman of Hellenic Quality Assurance and Accreditation Agency (HQAA), Greece and an Adjunct faculty at the International Center for Chemical and Biological Sciences (ICCBS), University of Karachi. Dr. Ioannis current research interest includes Bioorganic Chemistry, Natural Products, Medicinal Chemistry and Structural Biology. He has got distinction as Honorary Research Fellow, QMW College, Medal of the Ministry of Education of Ukraine and D.Sc. University of East Anglia. He has been honored with many Fellowships and Awards. He has over 140 publications in international journals with total impact factor ~ 500, number of citations 2652 and h-index 29 (Web of Science). He is the Reviewer of more than thirty (30) International journals including Analytica Chimica Acta, Chemical Biology, Chemical Physics Letters, European Journal of Biochemistry, FEBS Letters, Food Chemistry, Journal of the American Chemical Society, Journal of Chemical Education, Journal of Magnetic Resonance etc. Dr. Ioannis has also performed his duties as member of National and International Committees. He is the Member of Editorial Advisory Board of the Journals “The Open Natural Products Journal”, “International Journal of Green Pharmacy”, “ISRN Spectroscopy”. He is the Author/Editor of Educational Books of “Spectroscopy of Organic Compounds” and “Organic Chemistry”. He is also Author/Editor of Research Book namely “Natural Antioxidant Phenols. Sources, Structure, Structure – Activity Relationship, Current Trends in Analysis and Characterization”
Course Content
Basic concepts of NMR
Students will be able to:
- Know how nuclear spins are affected by a magnetic field, and be able to explain what happens when radiofrequency radiation interacts with nuclei.
- Understand the physicochemical basis of H 1 , 13C and heteronuclear chemical shifts and their relation to structure and conformational analysis.
- Understand coupling constants and their relation to structure and conformational analysis.
- Understand the fundamental theory of 1- dimensional Fourier Transform NMR.
- Understand the various NMR relaxation mechanisms.
- Understand the basics of Nuclear Overhauser Effect (NOE) and its use in structural analysis.
- Understand the basics of NMR applications in dynamics and chemical exchange phenomena.
Biomolecular NMR spectroscopy
Students will be able to understand:
- The assignment problem and the basic strategy to assign resonances.
- The chemical shift ranges of peptides and proteins.
- The fundamental theory of two- dimensional NMR spectroscopy and how to add an extra dimension.
- The sequential assignment strategy and its use in structure determination.
- The use of heteronuclear multi-dimensional NMR.
- The optimum experimental conditions used.
- The basics in NMR analysis of protein dynamics.
- The basics of protein-ligand interactions
NMR of DNA and RNA
Students will be able to understand:
- The nucleoside and nucleotide spin – systems.
- The chemical shift ranges of nucleic acids.
- The sequential assignment method.
- The use of distance constraints NMR in structural analysis.
- The use of NMR in investigating base pair life times and dynamic phenomena.
NMR of biomolecules in the solid state
Students will be able to understand:
- The basics of anisotropic interactions.
- The effects of magic angle spinning, cross-polarization and most essential techniques in solid state NMR.
- Use of NMR in investigating polymorphs and natural products.
- Use of NMR in investigating protein structures.
- The basic experimental conditions
NMR metabolomics
- The basic principles of metabolomics.
- Basic NMR techniques used in metabolomics.
- Basic experimental conditions.
- Selective applications.
NMR in plant metabolomics
- The basic principles in plant metabolomics.
- Basic NMR techniques used in plant metabolomics.
- Basic experimental conditions.
- Selective applications.
Case studies in natural products
- The basic concepts of in cell NMR” in natural products.
- How to obtain high resolution NMR structures: toward NMR “crystallography” in solution. i. The case of hydrogen bonding. ii. Chemical shift constrains in high resolution structures. iii. The hypericin and hexacyclinol dispute. iv. On the stability of natural products in aqueous solution.
- Principles of computational analysis of complex 2D NMR spectra of mixtures of natural products.
Lectures Schedule
Registration
For registration, kindly contact VEPP Focal Person in your University. The L. E. J. Center will issue a certificate (1 credit hr) to successful participants.
Click Here to find focal person in your university.
Event Title: Online Course: NMR Spectroscopy of Biological Molecules
Event Date: April 19, 2016
Event Venue: Video Conferencing Studios / Rooms of Participating Universities AND Video Conferencing Facility, L.E.J National Science Information Center, ICCBS, University of Karachi, Karachi, Pakistan
For Correspondence
Dr. Sheeba Naz
Manager
Virtual Education Project Pakistan (VEPP)
UAN: 111-222-292 Ext 274, 275
Email: [email protected]
